Knowledge-enriched characterization of cell populations. Morphological information and facts from images can include on the richness of flow cytometry details. For example, the determine exhibits an normally typical apoptosis assay working with Annexin V and PI, adding cell imaging to characterize cells in Every populace to expose morphologically distinctive functions. These insights could not happen to be attained from multiplex staining by itself.

Regardless of whether you’re a novice or an expert, examine our assets to advance your research and expertise in flow cytometry.

Merge acoustic focusing technologies with superior-parameter Assessment for flexible spectral unmixing and standard workflows.

The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing by using a substantial-pace brightfield camera for simultaneous higher throughput flow cytometry and superior resolution brightfield imaging.

"An image isn't really pretty truly worth a thousand dots, but it really genuinely helps you to see photographs. It is great to discover images that correlate with staining. One example is, AnnexinV-optimistic cells which can be morphologically different than balanced cells, or CD14+ monocytes which might be greater and more textured than CD3+/CD4+/CD14- lymphocytes.

In addition, it is actually illuminating to determine the quantity of doublets slip as a result of classic singlet gates, and terrific to verify the status of unexpected double-favourable occasions in my analysis."

Pair your Attune flow cytometer having an autosampler for enhanced workflow performance, and add robotic integration for max throughput.

Dependable impression good quality even at higher throughput Acoustic focusing along with a superior-pace digital camera Incorporate to graphic these T cells continually at small or substantial flow prices. Conveniently adjust aim and digicam options to meet experimental requirements.

The Attune NxT cytometer may be the reliable workhorse instrument your lab can count on for regular and dependable effectiveness. The Attune CytPix design check here contains a superior-pace brightfield digicam and AI-driven image Assessment program, with latest updates which includes equipment learning resources and an intuitive person interface, enabling enhanced knowledge precision, accuracy, and speedier insights into sample biology.

Potentially most importantly, these situations comprise cells of clearly diverse phenotypes which may lead to incorrect conclusions relating to double positive events (especially in rare populations).

Sound waves empower specific mobile alignment, resulting in Outstanding facts top quality and ~10X speedier sample flow premiums.

Application Be aware: Automatic picture analysis cuts down user-to-consumer variability in flow cytometry gating strategies

Designed-in dimensionality reduction tools amplified resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to confirm predicted populace resolution in lysed entire human blood. Consumer can look at situations from Every cluster for Visible confirmation of cell phenotypes.

No clean/no lyse leukocyte Examination with imaging. Cells were obtained from 24-hour-aged blood by dilution in 1 mM EDTA ( Expert UX/UI allows customers to train AI models for expanded apps. (A) The “Coach Product” characteristic supplies annotation instruments and authentic-time design education accuracy.

Gating on these extended parameters allows you to immediately and properly determine populations of curiosity and confirm gating method with little if any guide review. The Attune computer software delivers access to condition-of-the-art equipment Understanding Evaluation equipment inside an expertly created consumer interface, making it exceptionally simple to operate.

As the mobile line experienced Earlier been analyzed over the Attune CytPix Flow Cytometer, the scientists went again to the pictures and were being ready to document the microbial infection at the very least five times previously.

The Attune CytPix Flow Cytometer will allow consumers to visualize samples all through acquisition, validate the precision of gating approaches, and assist effects with dependable documentation for publication reasons.

What seemed to be a homogeneous population is actually extra assorted—an Perception that needs to be deemed when interpreting outcomes.